The growth inhibitory components were in the methylene chloride soluble fraction of the extract. Concentrationresponse curves for the cytotoxic effect of. Plant extracts generally contain flavonoids in glycosidic form. The cytotoxic effect of the mo extracts was dependent on the cell type. A correlation has been observed between phenolic content of leaf and flower extracts and the antioxidant activity. The extract from carrot was more heatstable than other extracts. The screening of plant extracts has been of great interest to scientists in the search for new everal diseases. This concentration of dmso did not affect cell viability. In this study we investigated of antibacterial and synergistic effect of ficus sycomorus.
Preliminary phytochemical screening, quantitative estimation. The effect of plant extracts on the growth of wilt causing. Dry powder was extracted with ethanol for about 7 days at room temperature. Qualitative and quantitative phytochemical analysis of. To investigate the cytotoxic effect of some bangladeshi medicinal plant extracts, 16 bangladeshi medicinal plants were successively extracted with n hexane, dichloromethane, methanol and water.
Pdf the anthelminthic efficacy of some differently obtained extracts of several plants was tested in vivo in laboratory animals and in vitro. Foundation and perspectives of the use of plant extracts as. Can a plant extract show activity at lower doseconcentration but not. The sycamore fig belongs to family moraceae table 1 is one of the old and historic plant species in the palestine coastal valley and the study area as well. Pdf cytotoxic activity of a plant extract on cancer cells. Cytotoxic effects of bangladeshi medicinal plant extracts. Pdf the effects of different plant extracts on nematodes. Antioxidant properties of the methanol extracts of the leaves. The plant sources of india are likely to provide effective anticancer agents. Biological screening of various medicinal plant extracts for. Cytotoxic activity of extracts from plants of central. Materials and methods the pure culture of the pathogen isolated from ripened.
The antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method. Cytotoxic and apoptotic effects of different extracts of. The plant materials were air dried at room temperature for about 10 days and grounded into powder. Efficacy of plant extracts on the germination of wheat seeds. Herbs, spices, plant extracts and essential oils eo have been used for centuries to preserve food for humans and to make it more appetizing. Pdf potential genotoxic and cytotoxic effects of plant. Evaluation of some plant extracts for their nematicidal. These data indicate that selected plant extracts are potential sources of dietary antioxidants. Herbs have a vital role in the prevention and treatment of cancer. In addition, the possible synergistic effects when associated with antibiotics were studied.
Antioxidant and in vitro cytotoxic activity of extracts of. Nov, 2014 the effect of plant leaf extract incorporated feed for the disease resistance survival percentage on fish n 20group were determined. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. With a view to identify effective plant extracts against colletotrichum capsici, present investigations were undertaken during kharif, 2005 in laboratory with the objective, to study effect of plant extracts for antifungal properties. The antimicrobial activity of 12 plant extracts was tested against the phytopathogens alternaria alternata and fusarium solani. Extraction, isolation and characterization of bioactive compounds. The highest values of plant height, stem diameter, dry weight of leaves plant, leaf area, total carbohydrates and n contents were obtained with. Bello abstract phytochemicalsare active secondary plant metabolites responsible for most of the claimed medicinal activities of plants. Therefore, in vivo, the mode and site of action of the active components of essential oils depend on their structure, metabolism, and inclusion level brugalli, 2003. The results revealed that the extracts of the two plant species caused a considerable reduction in the number of weevils. The present study aimed to assess the cytotoxic activity of extracts of selected plant species used for treatment of malaria in tanzania. Many studies have demonstrated antiproliferative, cytostatic and cytotoxic activities of phytochemicals against cancer cells 2. Pdf cytotoxic activities of extracts of medicinal plants. Plant phenolics are generally involved in defense against ultraviolet radiation or aggression by pathogens, parasites and predators, as well as contributing to plants colors.
Synergistic effect of ficus sycomorus moraceae leaf and. Increasingly, natural products have potential for the treatment of cancer. Assessment of antioxidant activity of plant extracts by. The three extracts stored in the dark at 5 and 25 c after a 15 day period did not show any significant change p. The present study deals with the analysis of phytochemical constituents by qualitative and quantitative analysis of moringa concanensis leaves, flowers and seeds were done using methanol extract. Evaluation of some plant extracts for their nematicidal properties against rootknot nematode, meloidogyne incognita m. The bioactive proteinpeptide extracts were found more free radical scavengers than the crude extracts of seeds and. They are ubiquitous in all plant organs and are therefore an integral part of the human diet. Alcoholic or hydroalcoholic extracts from plant principles in which 1 ml of fluid extract is obtained from 1g of plant, e. Effect of humic acid and seaweed extracts on growth and yield of potato plant solanum tubersum l desiree cv. The analysis of bioactive compounds present in the plant extracts involving.
In spite of this, the effectivity classification of the inhibition of phenolics and the extracts remained the same. Flowers and leaves extracts showed a powerful antimicrobial effect against all strains and the most. In order to extract different phenolic compounds from plants with a. Thereafter, we studied the extraction of polyphenols and the antioxidant activity of. The flower extracts of these two species were found to have higher cytotoxic effect on mcf7 cell with an ic50 value of 221.
Saha 1department of agricultural chemistry, hajee mohammad danesh science and technology university, dinajpur, bangladesh. Phenolics are widespread constituents of plant foods fruits, vegetables. Effect of some natural extracts on growth and chemical. It is difficult to distinguish between the different eo available, as each eo is. Dry ethanolic extracts were obtained after removing the solvent by evaporation. Effect of crude plant extracts from some oaxacan flora on two. Baskaran abstract nematicidal activities of extracts from plants were assayed against meloidogyne incognita. The extract was orally administered at 50 and 200 mgkg body weight. Chemoprevention by natural products may be considered a promising approach to cancer control and management 1. Total phenolic content was found to be higher in leaf extracts except c.
The study resulted in nonmutagenic and negligible cytotoxic plant extracts. Cytotoxic properties of some medicinal plant extracts from. Dilution of stock solutions was made in culture medium yielding final extracts concentrations with a final dmso concentration of 0. Antimicrobial effects of finnish plant extracts containing. The aim of this study is to determine the antifungal activity and phytochemical composition of the plants extracts.
This value is the threshold established by the national cancer institute usa, nci plant screening program for an extract to be considered active following an incubation period of 48 and 72 h 52. Phytochemical composition of ethanolic and aqueous extracts of the leaves, stembark, root, fruits and seeds i. The trees have some medicinal values as the sap extracted from the trunk. Effects of medicinal plant extracts and photosensitization on. In practice, plant extracts should be included in feeds at much higher doses to produce the same bactericidal or bacteriostatic effect observed in vitro.
The diluted working solutions of the test extracts were prepared in methanol. The effect of various plant extracts was measured on several important oxidation indicators. Phytochemical screening of some compounds from plant leaf. Alkaloids, flavonoids, terpenoids, carbohydrates, protein and amino acids were analysed. In screening for genotoxic and cytotoxic effects, extracts of different plant parts have been used, ranging from leafy vegetables, fruits, and underground storage organs to whole plants. Syzygium cordatum mean 3 cfus had the lowest cfu reading at 40 minutes and hence was the. The current study included the preparation of the aqueous extract and alcohol leaves ziziphus ziziphus spina christi, eucalyptus plant. Juss were evaluated for their growth, adult mortality and oviposition inhibition of c.
Cytotoxic studies of plant extracts on cancer cell lines zerumbone inhibited the proliferation of human colonic adenocarcinoma cell lines ls174t, ls 180, colo205 and colo320 dm in a dose dependent manner, while the growth of normal human dermal 2foc25 and colon ccd18 co fibroblasts was less affected. This study evaluated the effect of growth substrates and plant extracts at different concentrations on germination and incidence of disease in pinus tecunumanii plants. Evaluation of the cytotoxic activity of extracts from. The plant aqueous, ethanolic, acetone and methanolic extracts were screened for the presence of the phytochemical classes by using the standard following methods. Now, the international to research plant active ingredients of cosmetics are in full swing, united states, japan, france, as well as some wellknown chinese cosmetic makers were add a variety of natural plant extracts in cosmetics, such as panax ginseng root extract, aloe vera extract, almond extract, polygonum multiflorum extract, ginkgo. Antimicrobial and antioxidant activity of leaf and flower. Effect of aqueous plant extracts and photosensitization on a. Phytochemicals, antioxidant and antimicrobial potentials. The fish were artificially challenged with dose of 1. Effect of aqueous and alcoholic plant extracts on inhibition of some. Pdf effects of herbal plant extracts on germination and. Effects of herbal plant extracts on germination and seedling growth of some vegetables article pdf available september 2015 with 1,822 reads how we measure reads. Antiinflammatory and analgesic activity of methanolic. International journal of plant sciences, antifungal.
The effect was greater in the light than in the dark. Could it be inhibitory compounds in the plant extracts that inhibits cells growth. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. Nov 05, 20 the cells were treated with different concentrations of plants extracts including 0. This may be the reason why the plant extracts did not produce as marked inhibition as many of the pure compounds. A marked antimicrobial and antioxidant activity of leaf and flower extracts was observed. Pdf cytotoxic activities of certain medicinal plants on. Potential genotoxic and cytotoxic effects of plant extracts. In vitro inhibitory activities of selected australian medicinal plant. These activities have been corelated to the presence of certain phytochemical substances. These products are well recognized for their health benefits in many societies around the world. Plant growth regulatory effect and insecticidal activity of. Ten dif ferent plants were collected from in and around sivakasi area.
Cytotoxic activity of a plant extract on cancer cells. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Green plants extracts innovative botanical and herbal. Morphine was administered subcutaneously at a dose of 5mgkg body weight 10. Estimation of the total flavonoids in the plant extracts was carried out using the method of ordon ez et al. Antioxidant activity was determined by measuring the scavenging effect of plant extracts against 1, 1diphenyl2picryl hydrazyl dpph and using the ferric. Ethanol extracts were evaluated for cytoxicity by using mtt assay on llcmk2 cells and by brine shrimp lethality assay. Screening of invitro hypoglycemic activity of murraya koenigii and. Therefore, plant oils are widely used as a substrate for testing antioxidant activity.
Evaluation of antioxidant activity of some plant extracts and. The leaf extracts of romatic plants, murraya koenigii and azadirachta a. A yellow color indicated the presence of flavonoids. The plants were inoculated with the dampingoff pathogen fusarium oxysporum and treatments were applied in a completely randomized design with a factorial arrangement of 4x2x3.